Dilution Factor Calculator - Calculate Laboratory Dilutions with Step-by-Step Solutions
Free dilution factor calculator. Calculate dilution factors, concentrations, and volumes for laboratory solutions with step-by-step solutions. Our calculator uses laboratory principles to determine all dilution relationships from any given inputs.
Last updated: October 19, 2025
Need a custom laboratory calculator for your educational platform? Get a Quote
10
Dilution ratio
10 mg/mL
Final concentration
10 mL
Solution volume
100 mg/mL
Original concentration
Given stock concentration: 100 mg/mL
Given final concentration: 10 mg/mL
Dilution factor = Stock / Final
Dilution factor = 100 / 10
Dilution factor = 10
Volume needed: 10 mL
Serial Dilution
Sequential dilution steps
Parallel Dilution
Single dilution step
Concentration
Concentration-based calculation
Applications:
- • Laboratory work
- • Chemical analysis
- • Biological assays
- • Quality control
Common Dilutions
Practical Examples
Dilution Factor Calculator Types & Features
Formula used
DF = Stock / Final
Calculates dilution factor from concentrations
Formula used
Final = Stock / DF
Calculates final concentration from dilution factor
Formula used
V = (C_f × V_f) / C_s
Calculates volume of stock solution needed
Method used
Sequential Steps
Multi-step dilution calculations
Method used
Single Step
Direct dilution from stock solution
Features
Complete Analysis
Comprehensive laboratory calculations
Quick Example Result
For stock 100 mg/mL to final 10 mg/mL:
Dilution Factor
10
Final Conc.
10 mg/mL
Volume
10 mL
Stock Conc.
100 mg/mL
How Our Dilution Factor Calculator Works
Our dilution factor calculator uses the fundamental principles of laboratory dilution to calculate dilution factors, concentrations, and volumes from any given inputs. The calculation applies laboratory methods and dilution theory to determine all dilution relationships.
The Fundamental Dilution Formulas
Dilution Factor = Stock / FinalFinal Concentration = Stock / Dilution FactorVolume of Stock = (Final × Volume) / StockSerial Dilution = (DF per step)^nThese formulas form the foundation of laboratory dilution calculations and allow determination of all dilution parameters from any given values. They apply to both simple and complex dilution schemes.
Shows the dilution process from stock solution to final concentration
Laboratory Foundation
Dilution factor calculations are fundamental in laboratory work for preparing solutions of known concentrations. They are based on the principle of conservation of mass, where the amount of solute remains constant while the volume increases. This is essential for analytical chemistry, biochemistry, and molecular biology applications.
- Dilution factor represents the ratio of original to final concentration
- Serial dilutions use multiple sequential steps
- Parallel dilutions use single direct steps
- Volume calculations ensure accurate solution preparation
- Concentration calculations verify dilution accuracy
- All methods preserve the conservation of mass principle
Sources & References
- Analytical Chemistry - Skoog, West, Holler, CrouchComprehensive coverage of dilution and concentration calculations
- Laboratory Techniques in Organic Chemistry - Mohrig, Hammond, SchatzPractical laboratory methods for solution preparation
- Khan Academy - Dilution and ConcentrationEducational resources for understanding dilution calculations
Need help with other laboratory calculations? Check out our concentration calculator and molarity calculator.
Get Custom Calculator for Your PlatformDilution Factor Calculator Examples
Given Information:
- Stock concentration: 100 mg/mL
- Final concentration: 10 mg/mL
- Volume: 10 mL
- Goal: Calculate dilution factor
Calculation Steps:
- Given stock concentration: 100 mg/mL
- Given final concentration: 10 mg/mL
- Dilution factor = 100 / 10 = 10
- Volume needed: 10 mL
- Result: 10-fold dilution
Result: Dilution Factor = 10 (10-fold dilution)
To prepare 10 mL of 10 mg/mL solution from 100 mg/mL stock, use 1 mL of stock + 9 mL of diluent.
Serial Dilution
3 steps of 1:10 each
Total dilution: 1:1000
Volume Calculation
Stock 50 mg/mL, final 5 mg/mL, volume 20 mL
Volume needed: 2 mL
Frequently Asked Questions
Found This Calculator Helpful?
Share it with others who need help with laboratory dilution calculations
Suggested hashtags: #Lab #Chemistry #Dilution #Concentration #Education #Calculator